Nitrofuran SEM Diagnostic ELISA Kit for Shrimp 96 Wells/Kit
Sensitivity 0.02
1. Nitrofuran SEM Diagnostic ELISA Kit Principle
This test kit is based on a competitive enzyme immunoassay for the
detection of nitrofurans (SEM) in samples. Conjugated antigens are
pre-coated on microwell strips. The nitrofuran (SEM) in the sample
competes with the bound antigen pre-coated on the microwell strip
for anti-SEM antibodies. After adding the enzyme conjugate, add TMB
substrate for staining. The optical density (OD) value of a sample
is negatively correlated with the SEM in it. This value was
compared to a standard curve and the SEM concentrations were
subsequently obtained.
2. Technical specifications
Sensitivity: 0.02ppb
Incubation Temperature: 25℃
Incubation Time: 30min～15min
Detection limit Tissue, egg, honey: 0.1ppb
Detection limit Feed:4ppb
Cross-reaction rate:
SEM 100%
AMOZ less than 0.1%
AOZ less than 0.1%
AHD less than 0.1%
Recovery rate:
Tissue 75±25%
Honey 70±20%
Egg 95±25%
3. Nitrofuran SEM Diagnostic ELISA Kit Components
| 1 | Micro-well strips | 12 strips with 8 removable wells each |
| 2 | 6× standard solution (1 mL each) | 0ppb | 0.02ppb |
| 0.06ppb | 0.18ppb |
| 0.54ppb | 1.62ppb |
| 3 | Enzyme conjugate | 7ml | red cap |
| 4 | Antibody working solution | 7ml | blue cap |
| 5 | Substrate A | 7ml | white cap |
| 6 | SubstrateB | 7ml | black cap |
| 7 | Stop solution | 7ml | yellow cap |
| 8 | 20× concentrated washing buffer | 40ml | white cap |
| 9 | 2× concentrated redissolving solution | 50ml | transparent cap |
| 10 | 2-Nitrobenzaldehyde (C7H5NO3) | 10ml | black cap |
4. Samples preparation
Feed
1) Weigh 1± 0.05g of the homogenized sample, add9mL of the50%
Ethanol absolute,then add5mL n-Hexane, fully shake for 3min;
2) Centrifuge at above 4000r/min at room temperature (20-25 ℃) for
10min;
3) Take5mL middle-layer water phase into another50mL clean
centrifuge tube, add0.5mL 1 M HCI and 100µL 2-Nitrobenzaldehyde
(C7H5NO3),fully shake for 2min;
4) Incubate at 70℃ by water bath for 20 minutes;
5) Add 5mL 0.1M K2HPO4, 0.4mL 1M NaOH and10mL ethyl
acetate,fullyshake for 30s;
6) Centrifuge at above 4000r/min at room temperature (20-25 ℃) for
10min;
7) Transfer1mL oftheethyl acetate layer into a newcentrifugal
tubeand evaporate to dryness by nitrogen or air at 50℃.
8) Dissolve the dry residues in2mL N-hexane, add 1mL of the diluted
redissolving solution, mix properly for 30 seconds, centrifuge at
above 4000 r/min at room temperature (20-25 ℃) for 10 min; Remove
up-layerN-hexane phase;
9) Take 200µl down-layer clear liquid and 800µlof the diluted
redissolving solution, mix it evenly;
10) Take 50 µLliquid for analysis.
Fold of dilution of the sample:100
5. Matters needing attention
1) If the room temperature is lower than 25℃ or the reagent
temperature and the sample do not return to room temperature
(20-25℃), the standard OD value will drop.
2) During the washing process, the drying of the microplate will be
accompanied by the nonlinearity of the standard curve, and the
reproducibility is not ideal; therefore, the next step should be
performed immediately after washing.
3) Mix well, otherwise the reproducibility is poor.
4) The stop solution is 2 M sulfuric acid solution, avoid skin
contact.
5) Do not use if the kit has expired. The use of diluted or
adulterated reagents from the kit can result in changes in
sensitivity and detection OD values. Do not replace reagents in
different batches of kits.
6) Reseal unused microplates in ziplock bags. Standard solutions
and colorless couplers are sensitive to light and cannot be
directly exposed to light. Please store them in the dark.
7) Discard any coloring solution whose color indicates that the
solution has degraded. A detection value of less than 0.5 for
Standard Solution 1 (0 ppb) indicates degradation.
8) The optimal reaction temperature is 25℃, and the detection
sensitivity and OD value will change if the temperature is too high
or too low.
6. Storage and validity period
Storage: Store at 2-8°C, do not freeze.
Expiration date: 12 months; production date is on the box.
Note: If the microplate vacuum packaging leaks, it can still be
used without affecting the test results, please use it with
confidence.
Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7
working days after receiving the payment. (In case of external
factors such as the epidemic, the delivery may be delayed)
Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more
than 100,000 pieces, which is convenient for customized products.
Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our
production process conforms to standard procedures to ensure
optimum product quality.
Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service.
We can provide you with one-on-one guidance via video, telephone,
etc.
Q5: What is the payment method?
A5: We receive payment by T/T.
Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from
our many cooperative carriers, and also ship according to your
requirements.
