Deoxynivalenol Diagnostic ELISA Kit for Feed 96 Wells/Kit
Sensitivity 2ppb
1. Deoxynivalenol Diagnostic ELISA Kit Principle
This kit is based on the indirect competitive enzyme immunoassay
for the detection of deoxynivalenol (DON). Conjugated antigens are
pre-coated on microwell strips. Deoxynivalenol (DON) in the sample
competes for anti-deoxynivalenol (DON) antibodies with the
conjugated antigen pre-coated on the microwell stripes. After the
enzyme conjugate is added, TMB substrate is added for staining. The
optical density (OD) value of the sample was negatively correlated
with deoxynivalenol (DON) in the sample. This value was compared to
the standard curve and the deoxynivalenol (DON) residue was
obtained.
2. Technical specifications
Sensitivity: 2ppb
Incubator temperature: 25℃
Incubator time: 30min~15min
Detection limit:
| Feed | 300ppb |
| Rice, maize | 100ppb |
| Peanut | 50ppb |
Cross-reaction rate:
Deoxynivalenol(DON) 100%
Recovery rate:
| Feed, rice, maize | 90±30% |
| Peanut | 100±30% |
3. Deoxynivalenol Diagnostic ELISA Kit Components
| 1 | Micro-well strips | 12 strips with 8 removable wells each |
| 2 | 6× standard solution (1 mL each) | 0ppb | 2ppb |
| 6ppb | 18ppb |
| 54ppb | 162ppb |
| 3 | Enzyme conjugate | 7ml | red cap |
| 4 | Antibody working solution | 7ml | blue cap |
| 5 | Substrate A | 7ml | white cap |
| 6 | Substrate B | 7ml | black cap |
| 7 | Stop solution | 7ml | yellow cap |
| 8 | 20× concentrated washing buffer | 40ml | white cap |
| 9 | 2× concentrated redissolving solution | 50ml | transparent cap |
4. Matters needing attention
1. The standard OD value will be low if the room temperature is
lower than 25℃ or the reagent temperature and the sample has not
returned to room temperature (20-25℃).
2. The microplate is dried during the washing process, which will
be accompanied by non-linearity of the standard curve and poor
reproducibility; therefore, proceed to the next step immediately
after washing.
3. Mix well before adding any reagents.
4. The stop solution is 2M sulfuric acid solution, avoid contact
with skin.
5. Do not use the kit beyond the expiration date. The use of
diluted or adulterated reagents from the kit can result in changes
in sensitivity and detection OD values. Do not replace reagents in
different batches of kits.
6. Storage: Store at 2-8°C, do not freeze. Reseal unused
microplates in self-sealing bags. Standard materials and colorless
couplers are sensitive to light and cannot be directly exposed to
light.
7. Discard any tinting solution whose color indicates that the
solution has degraded. The detection value (450/630nm) of 0
standard solution (0 ppb) is less than 0.5 ((A450nm<0.5))
indicating its denaturation.
8. The optimal reaction temperature is 25℃. If the temperature is
too high or too low, the detection sensitivity and OD value will
change.
5. Storage and shelf life
Storage: Store at 2-8°C, do not freeze.
Expiration date: 12 months; production date is on the box.
Note: If the microplate vacuum packaging leaks, it can still be
used without affecting the test results, so you can use it with
confidence.
Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7
working days after receiving the payment. (In case of external
factors such as the epidemic, the delivery may be delayed)
Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more
than 100,000 pieces, which is convenient for customized products.
Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our
production process conforms to standard procedures to ensure
optimum product quality.
Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service.
We can provide you with one-on-one guidance via video, telephone,
etc.
Q5: What is the payment method?
A5: We receive payment by T/T.
Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from
our many cooperative carriers, and also ship according to your
requirements.
