Anti-Japanese Encephalitis Virus Antibody Rapid Test Kit 50
Tests/Kit GMP
1. Principle
This rapid test is an immuno-chromatographic test, using colloidal
gold immunoassay method, to detect the antibody against Japanese
encephalitis virus in whole blood, serum. After adding sample, the
sample move along with the colloidal gold labeling JEV antigen, is
there is JEV antibody in the sample, it combines with antigen on T
line and show wine-red color; if there is no JEV antibody, then no
color reaction.
2. Components
| 1 | JEV Ab test card | 50 pieces |
| 2 | Dropper | 50 pieces |
| 3 | Antibody titers Colorimetric card | 1 piece |
| 4 | Disposable gloves | 1 pair |
| 5 | Manual | 1 piece |
3. Test procedure
1) Collect whole blood, separate serum (centrifuge at 2000-3000
r/min for 5~15min or separate out naturally at 4℃overnight), or
take whole blood with anticoagulants as sample. Take whole blood
without anticoagulants as sample is also OK, but must test it
immediately.
2) Open the package, take out test card, put it on clean, flat
desk.
3) Use dropper to absorb the sample, adding 3 drops sample into “S”
mark well slowly drop by drop.
4) Judge the result in 10-20min, it is invalid beyond 20min.
4. Results judgment
1) Positive: control line and test line are both seen wine red, the
more the antibody exist, the thicker the color appear;
2) Weak positive: control line and test line are both seen wine
red, but the color of test line is very light;
3) Negative:only control line is seen wine red;
4) Invalidation: control line isn’t seen wine red.
5. Consults for diagnosis
1) If not vaccinated against porcine JEV virus:
One. There is no visible wine red on the test line, indicating that
there is no antibody against the pig JEV virus in the test sample.
If the pig is healthy, it is best to vaccinate in time;
b. Visible wine red on the detection line indicates that the pig is
infected with wild virus, and more observation and verification
should be made.
2) If vaccinated against porcine JEV virus:
One. The color of the detection line is 1:40 darker than the color
of the titer. The control card shows that the antibody titer
against the swine JEV virus is high enough to resist the strong
virus;
b. The color of the test line is thinner than the color of the
titer. The 1:40 comparison card shows that the antibody titer
against swine JEV virus is not sufficient against strong virus, and
supplementary vaccination is better.
6. Storage and expiry date
Store at 2 - 30ºC and the shelf life is 24 months.
7. Notice
1) Do not use expired or poorly packaged test cards;
2) Do not use purified water, tap water and normal saline as
negative controls;
3) Store at room temperature (< 30°C). If stored in a cool
place, you need to return to normal temperature before opening the
package to avoid moisture. Use the test card as soon as possible
after opening the package.
4) Do not touch the white film surface in the middle of the test
card.
5) To ensure the quality of the test, please take fresh,
non-contaminated samples. If there are too many blood lipids, it
will affect the results, so after centrifugation, do a serum test
to avoid gaining weight. If the blood or serum is viscous or not
viscous enough, it can be diluted with normal saline to the same
dilution before testing.
6) If serum cannot be detected immediately, it can be stored at 4°C
for a short time (within 48 hours) and at -20°C for a long time.
7) Whole blood without anticoagulant should be tested immediately;
if some Krull is already present, separate serum for testing. Whole
blood containing anticoagulants should be tested within 24 hours
and should not be frozen. Freezing and thawing lead to hemolysis
and rupture of red blood cells, which seriously affects the test
results.
8) When adding the sample dropwise, keep the pipette tip 1cm from
the "S" hole to ensure accurate sample addition. If it is too
close, the droplet volume will be too small, which will lead to
wrong judgment of the droplet or not allow the amount of sample to
be added, which will affect the test results.
9) Avoid foaming when adding samples, and the sample volume should
be controlled at about 100ul (about 3 drops). Too many samples will
not increase sensitivity. In particular, too much whole blood can
cause red blood cells to settle in the "wells", causing membrane
chromatography to penetrate deep into the red background, seriously
affecting the results.
10) This test card is for pig samples only and its applicability to
other animals has not been evaluated.
11) Antibody levels are only related to T-line darkness, not
C-line. Only the C wire is clean, indicating that the test is
valid.
12) Dispose of test cards and samples at the contaminated site
according to local regulations.
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific
needs and specific quantities.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so
far all the production process, we have standard rules, we comply
with the relevant behavior and laws of the government.
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service.
If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
Q5: What is your minimum order quantity?
A5: 1Kit.
Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by
air and ground.Please confirm with us before placing an order.
