Florfenicol Rapid Test Strips for Tissue 96 Tests/Kit 0.1 ppb
1. Specifications:
8 dipsticks/bottle, 12 bottles/box
2. Brief
This product is used for testing Florfenicol in tissue sample
(including pork, chicken, fish, shrimp etc.) qualitatively. Easy to
operate, high sensitivity.
3. Detection limit
Tissue: 0.1mg/kg (0.1ppb)
4. Contents
| 1 | 8 microwells and 8 dipsticks in one bottle | 12 bottles / kit |
| 2 | Instruction | 1 piece |
| 3 | Sample Redissolving solution | 1 bottle |
5. Principle
The Florfenicol rapid test dipstick is based on competitive
inhibition immuno-chromatographic principle. In the flow process,
Florfenicol in the sample combined with Florfenicol specific
colloidal gold-labeled monoclonal antibody, Inhibit the combination
between antibody and Florfenicol-BSA conjugate on Test line of NC
membrane, lead to the color change of Test line. When the sample
has no Florfenicol residue or concentration lower than detection
limit, T line is darker than C line or T line has same color with C
line; when the concentration is equal to or higher than detection
limit, T line is obviously lighter than C line or T line has no
color. No matter whether there is Florfenicol residue in sample, C
line will appear, it means the test is valid.
6. Materials needed but not provided
Equipment: homogenizer, nitrogen-drying device (or hair dryer),
vortex, oscillator (optinal), centrifuge, measuring pipets, balance
(a sensibility reciprocal of 0.01 g).
Reagents: 50ml centrifuge tube, tips, ethyl acetate, n-Hexane.
7. Sample preparation
Tissue
1) Take 3±0.05g homogenized tissue sample into 50ml centrifuge
tube, add 3ml deionized water, then add 7ml ethyl acetate, shake by
hand or oscillator for 2min, centrifuge at 4000r/min for 5min;
2) Take 4ml supernatant into another centrifuge tube, blow to dry
at 56℃by Nitrogen or air (or use hair dryer).
3) Add 0.5ml n-Hexane, shake for 30s (or repeatedly blow and beat
until Re-dissolved), dissolve substance on tube wall and bottom
completely, then add 0.45ml Sample Redissolving solution, shake for
10s, be static or centrifuge at 4000r/min for 5min until solution
stratification, discard up-layer n-Hexane phase, absorb 200ul
down-layer solution for test.
8. Operation procedures
8.1 Read the instruction carefully before use. Return test
dipsticks and sample into room temperature.
8.2 Take bottles needed from the kit package, take out required
microwells and dipsticks, making proper marks. Please use these
test dipsticks within 1h. Seal the cap of the bottles, avoid
moisture.
8.3 Take 200ul of the test samples into the micro-wells, then
repeatedly absorb for many times, mix the sample with the reagent
in the micro-wells completely (this is a very important step).
8.4 Incubate for 5min at room temperature (20-25℃), insert the test
dipsticks into the micro-wells with the "MAX" end fully dipped in
to the mixture solution.
8.5 Insert the test dipsticks into micro-wells for 3-5min then read
the result, it is invalid in other time.
9. Test Result Interpretation
9.1 Negative: T line is darker than C line, or T line has same
color with C line. It means there is no Florfenicol residue in
sample or the residue is lower than detection limit.
9.2 Positive: T line is obviously lighter than C line or T line is
invisible. It means the Florfenicol residue is equal to or higher
than detection limit.
9.3 Invalidation: C line isn’t seen wine red. It means the test
card is out of efficacy, out of date or improper operation. Please
run the test again using another package. If the invalid tests keep
happening, please contact the supplier.
10. Precautions
1) The test dipsticks can be used only once at room temperature, do
not use test dipsticks out of expiry date.
2) Do not touch the white membrane surface in the middle of test
dipsticks, avoid sunlight and fan blowing directly.
3) Use the test dipsticks testing again for positive results.
4) Please contact the supplier for any questions.
11. Specificity
Test 500ppb Sulfonamides, quinolones and tetracyclines and other
drugs. All the results are negative.
12. Storage and expiry date
Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.
Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7
working days after receiving the payment. (In the event of the
epidemic and other external factors, there may be delays in
shipping)
Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more
than 100,000 pieces to facilitate customized products.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 and ISO13485 certified by the state. Our
production process is in accordance with the standard process,
which can ensure that the quality of the products is optimal.
Q4: How is after-sales service provided?
A4: We provide professional online technical after-sales service.
We can provide you with one-on-one guidance in the form of video,
phone calls, etc.
Q5: What is the payment method?
A5: We receive payment by T/T.
Q6: How to ship?
A6: By obtaining quotations from our many cooperative carriers,
choose the best way to ship for you, or you can ship according to
your requirements.
