Nitrofuran AHD Rapid Test Strip for Tissue 96 Tests/Kit 0.5 ppb
1. Specifications
96 tests/kit
2. Brief
This product is used for testing Nitrofuran(AHD) in Chicken,
Aquatic Products (Fish, Shrimp, Crab) sample qualitatively. Easy to
operate, high sensitivity.
3. Detection limit
Tissue: 0.5ng/g (0.5ppb)
4. Contents
| 1 | Nitrofuran(AHD) test dipsticks bottle(include test
dipsticks,desiccant, micro-wells reagent) | 12 bottles |
| 2 | Manual | 1 piece |
| 3 | 1M HCl | 2 bottles |
| 4 | 1M NaOH | 2 bottles |
| 5 | 1M K2HPO4 solution | 2 bottles |
| 6 | Derivatization reagent | 1 bottle |
| 7 | Sample buffer | 2 bottles |
5. Principles
Nitrofuran (AHD) rapid test strips are based on the principle of
competitive inhibition immunochromatography. During the flow
process, nitrofuran (AHD) in the sample binds to nitrofuran
(AHD)-specific colloidal gold-labeled monoclonal antibody,
inhibiting the antibody and nitrofuran (AHD)-BSA at the NC membrane
detection line Associates coupling agent. pair combinations,
resulting in a change in the color test line. When the sample has
no nitrofuran (AHD) residue or the concentration is lower than the
detection limit, the T line is darker than the C line or the T line
is the same color as the C line; when the concentration is equal to
or higher than the detection limit, the T line is obviously lighter
than the C line or the T line Lines have no color. The C line
appears regardless of whether nitrofuran (AHD) remains in the
sample, indicating that the test is valid.
6. Sample Preparation
6.1 Solution Preparation
0.1MK2HPO4 solution: Dilute 1M K2HPO4 solution 1:9 with deionized
water.
6.2 Sample Handling
Tissue:
1) Take 3±0.05g of homogenized tissue sample into a 50ml centrifuge
tube, add 6ml of deionized water, 750ul of 1M HCl and 300ul of
derivatization reagent, shake well for 2min, and take a 60°C water
bath for 30min;
2) Add 7.5ml 0.1M K2HPO4 solution, 600ul 1M NaOH and 10ml ethyl
acetate, shake well for 1min, and centrifuge at 4000r/min for
10min;
3) Take 5ml of supernatant in a glass tube and dry with nitrogen or
air at 56°C.
4) Add 2ml of n-hexane, shake well to dissolve the dry residue (if
it is not completely dissolved, the result may be unstable), then
add 0.6ml of loading buffer, shake well for 30s, stabilize to
separate layers, and then dry the upper layer (if there is an
emulsion) ), centrifuged at 4000r/min for 5min);
5) Aspirate 200ul of the lower liquid (water layer) to prepare for
testing. (Avoid absorbing upper liquid).
7. Operating procedures
7.1 Please read the manual carefully before use. Return the test
strip and sample to room temperature.
7.2 Take out the required bottle from the kit, take out the
required microwell and test paper, and make a mark. Please use
these test strips within 1 hour. Seal lid to avoid moisture.
7.3 Take 200ul of the sample to be tested in the microwell, absorb
it 5-10 times, mix the sample with the reagent in the microwell,
until the naked eye judges that there is no solid (this is a very
important step).
7.4 Incubate at room temperature (20-25°C) for 5 minutes, insert
the test paper into the microwell, and completely immerse the "MAX"
end in the mixed solution.
7.5 Incubate again at room temperature for 5-8min, read the result,
other time is invalid.
8. Interpretation of test results
8.1 Negative: T line is darker than C line, or T line and C line
are the same color. This means that there is no nitrofuran (AHD) in
the sample or is below the detection limit.
8.2 Positive: T-line is significantly brighter than C-line or
T-line is not visible. This means that the residual nitrofuran
(AHD) is at or above the detection limit.
8.3 Unqualified: No wine red color can be seen on the C line. This
means the test card is invalid, expired or mishandled. Please run
the test again with another package. If invalid tests continue to
occur, please contact the supplier.
9. Matters needing attention
1) The test card can only be used once at room temperature, please
do not use the expired test card.
2) Do not reuse tips to avoid cross-contamination.
3) Do not touch the white film surface in the middle of the test
card, avoid direct sunlight and direct fan blowing.
4) Wear gloves when testing.
5) If you have any questions, please contact the supplier.
10. Specificity
No cross-reactivity with nitrofurans AMOZ, AOZ and SEM.
11. Storage and expiration date
Storage: 2-8 ℃ dark, sealed, dry place, not frozen.
Expiry date: 12 months; production date is on the box.
Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7
working days after receiving the payment. (In case of external
factors such as the epidemic, the delivery may be delayed)
Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more
than 100,000 pieces, which is convenient for customized products.
Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our
production process conforms to standard procedures to ensure
optimum product quality.
Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service.
We can provide you with one-on-one guidance via video, telephone,
etc.
Q5: What is the payment method?
A5: We receive payment by T/T.
Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from
our many cooperative carriers, and also ship according to your
requirements.
